|
KMID : 1147720150080060281
|
|
Journal of Acupuncture and Meridian Studies
2015 Volume.8 No. 6 p.281 ~ p.287
|
|
|
Study of Mast Cells and Granules from Primo Nodes Using Scanning Ionic Conductance Microscopy
|
|
Yoo Yeong-Yung
Jung Goo-Eun
Kwon Hee-Min
Bae Kyoung-Hee
Cho Sang-Joon
Soh Kwang-Sup
|
|
|
Abstract
|
|
|
|
Acupuncture points have a notable characteristic in that they have a higher density of mast cells (MCs) compared with nonacupoints in the skin, which is consistent with the augmentation of the immune function by acupuncture treatment. The primo vascular system, which was proposed as the anatomical structure of the acupuncture points and meridians, also has a high density of MCs. We isolated the primo nodes from the surfaces of internal abdominal organs, and the harvested primo nodes were stained with toluidine blue. The MCs were easily recognized by their stained color and their characteristic granules. The MCs were classified into four stages according to the degranulation of histamine granules in the MCs. Using conventional optical microscopes details of the degranulation state of MCs in each stage were not observable. However, we were able to investigate the distribution of the granules on the surfaces of the MCs in each stage, and to demonstrate the height profiles and three-dimensional structures of the MCs without disturbance of the cell membrane by using the scanning ion conductance microscopy.
|
|
|
KEYWORD
|
|
|
acupuncture, histamine granule, mast cell, primo vascular system, scanning ion conductance microscope (SICM)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|